Improvement of double Ovsynch protocol by progesterone supplement in postpartum Holstein dairy cows | ||
| نشریه پژوهش در نشخوار کنندگان | ||
| Volume 11, Issue 3, November 2023, Pages 117-130 PDF (878.43 K) | ||
| DOI: 10.22069/ejrr.2023.21468.1903 | ||
| Authors | ||
| Mahdi Ansari* 1; Mohammad ali Eslami2; Mehdi Poorhamdollah3 | ||
| 1Department of Animal and Poultry Physiology, Gorgan University of Agricultural Sciences and Natural Resources. Gorgan. Iran | ||
| 2Domgostan-e kavir. Yazd. Iran | ||
| 3Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| Abstract | ||
| Background and objectives: The past few decades have seen a dramatic decrease in the fertility of dairy cows. Thus, reproductive performance became one of the main concerns in the dairy cow industry. Improvement of fertility increases the farmer’s income by reducing the culling rate, increasing milk production, and reducing the calving interval. Factors involved in reproductive management are multifactorial such as management, nutrition, and genetics. Synchronization protocols are one of the main management tools that have improved the reproduction of high-producing dairy cows. Nevertheless, the efficiency of these protocols is still not satisfying and the current study was carried out to improve the double Ovsynch protocol in postpartum dairy cows. Materials and methods: A total of 318 healthy postpartum dairy cows were used in the current study. Data of parity and milk yield (1st and 2nd months) were recorded for all cows. Briefly, on 40 DIM, cows were subjected to a double Ovsynch program and were checked for corpus luteum via ultrasonography on d 57, at the start of the 2nd Ovsynch. Cows without CL were divided into two groups including control (DO-CL, continuing the routine program), and DO+CIDR (including one progesterone supplement). Those bearing CL were considered as DO-CL receiving routine injections. All cows were checked for pregnancy via ultrasonography on d 32, 60, and 120 post-AI, and pregnancy per artificial insemination (P/AI) and pregnancy loss rate were calculated. Data were analyzed using the GLIMMIX Procedure of SAS software. Results: Cows bearing CL at the start of TAI Ovsynch, compared to DO-CL and DO-CIDR groups, had significantly higher P/AI on d 32 (50.84 vs. 29.27 and 41.46%, P = 0.03), 60 (45.76 vs. 21.95 and 36.58%, P = 0.01), and 120 (43.22 vs. 21.95 and 34.14%, P = 0.03) post-AI. Although cows with CL at the start of the TAI program had 15% lower pregnancy loss compared to the control group, this trait in the 2nd (P = 0.31) and 3rd-4th month (P = 0.75) was not significantly affected by treatments. Parity (primiparous vs. multiparous) had a significant effect on P/AI on d 32 (P = 0.04), but not on d 60 (P = 0.17) and 120 (P = 0.28) post-AI. The effect of milk yield (M ≥ 36 vs. M ˂36) on P/AI at d 32 (P = 0.003), 60 (P = 0.001), and 120 (P = 0.001) was significant. Dairy cows in M ˂36 group compared to M ≥ 36 had significantly higher P/AI on d 32 (52.96 vs. 35.63%), 60 (46.62 vs 28.11%), and 120 (44.41 vs. 26.08%) post-AI. The effects of treatment × parity (P = 0.41) or treatment × milk yield (P = 0.45) interactions were not significant. Conclusion: Improvement of P/AI via evaluating the presence of CL at the start of the TAI program and using a progesterone insert in cows without CL suggests an important role of progesterone during the growth of ovulatory follicles. Therefore, the identification of cows not bearing CL and applying progesterone is highly recommended. However, the difference between P/AI in cows that received CIDR compared to those in diestrous suggests that using a CIDR may not be sufficient and two CIDRs should be applied in order to achieve optimum results. | ||
| Keywords | ||
| Progesterone supplement; Ovsynch; dairy cows; estrous cycle; corpus luteum | ||
| References | ||
|
Astiz, S., and Fargas, O. 2013. Pregnancy per AI differences between primiparous and multiparous high-yield dairy cows after using Double Ovsynch or G6G synchronization protocols. Theriogenology, 79(7): 1065–1070. Barletta, R.V., Maturana Filho, M., Carvalh, P.D., Del Valle, T.A., Netto, A.S., Rennó, F.P., Mingoti, R.D., Gandra, J.R., Mourão, G.B., Fricke, P.M., Sartori, R., Madureira, E.H. and Wiltbank, M.C. 2017. Association of changes among body condition score during the transition period with NEFA and BHBA concentrations, milk production, fertility, and health of Holstein cows. Theriogenology, 104(2017): 30–36. Bisinotto, R.S., Chebel, R.C. and Santos, J.E.P. 2010. Follicular wave of the ovulatory follicle and not cyclic status influences fertility of dairy cows. Journal of Dairy Science, 93(8): 3578–3587. Bisinotto, R.S., Ribeiro, E.S., Lima, F.S., Martinez, N., Greco, L.F., Barbosa, L.F.S.P., Bueno, P.P., Scagion, L.F.S., Thatcher, W.W. and Santos, J.E.P. 2013. Targeted progesterone supplementation improves fertility in lactating dairy cows without a corpus luteum at the initiation of the timed artificial insemination protocol. Journal of Dairy Science, 96(4): 2214–2225. Borchardt, S., Pohl, A., Carvalho, P.D., Fricke, P.M. and Heuwieser, W. 2018. Short communication: Effect of adding a second prostaglandin F2α injection during the Ovsynch protocol on luteal regression and fertility in lactating dairy cows: A meta-analysis. Journal of Dairy Science, 101(9): 8566–8571. Burke J.M., sal Sota, R.L., de la Risco, C.A., Staples, C.R., Schmitt, E.J. and Thatcher, W.W. 1996. Evaluation of timed insemination using a gonadotropin-releasing hormone agonist in lactating dairy cows. Journal of Dairy Science, 79(8): 1385–1393. Cardoso Consentini, C.E., Wiltbank, M.C. and Sartori, R. 2021. Factors that optimize reproductive efficiency in dairy herds with an emphasis on timed artificial insemination programs. Animals, 11(2): 1–30. Cerri, R.L.A., Chebel, R.C., Rivera, F., Narciso, C.D., Oliveira, R.A., Amstalden, M., Baez-Sandoval, G.M., Oliveira, L.J., Thatcher, W.W. and Santos, J.E.P. 2011. Concentration of progesterone during the development of the ovulatory follicle: II. Ovarian and uterine responses. Journal of Dairy Science, 94(7): 3352–3365. Cerri, R.L.A., Rutigliano, HM., Bruno, R.G.S. and Santos, J.E.P. 2009. Progesterone concentration, follicular development and induction of cyclicity in dairy cows receiving intravaginal progesterone inserts. Animal Reproduction Science, 110(1-2), 56–70. Crowe, M.A., Hostens, M. and Opsomer, G. 2018. Reproductive management in dairy cows - The future. Irish Veterinary Journal, 71(1): 1–13. Dirandeh, E., Roodbari, A.R. and Colazo, M.G. 2015. Double-Ovsynch, compared with presynch with or without GnRH, improves fertility in heat-stressed lactating dairy cows. Theriogenology, 83(3): 438–443. Echternkamp, S.E., Thallman, R.M., Cushman, R.A., Allan, M.F. and Gregory, K.E. 2007. Increased calf production in cattle selected for twin ovulations. Journal of Animal Science, 85(12): 3239–3248. Endo, N., Nagai, K., Tanaka, T. and Kamomae, H. 2012. Comparison between lactating and non-lactating dairy cows on follicular growth and corpus luteum development, and endocrine patterns of ovarian steroids and luteinizing hormone in the estrous cycles. Animal Reproduction Science, 134(3-4): 112–118. Fuenzalida, M.J., Fricke, P.M. and Ruegg, P.L. 2015. The association between occurrence and severity of subclinical and clinical mastitis on pregnancies per artificial insemination at first service of Holstein cows. Journal of Dairy Science, 98(6): 3791–3805. Giordano, J.O., Thomas, M.J., Catucuamba, G., Curler, M.D., Wijma, R., Stangaferro, M.L. and Masello, M. 2016. Effect of extending the interval from Presynch to initiation of Ovsynch in a Presynch-Ovsynch protocol on fertility of timed artificial insemination services in lactating dairy cows. Journal of Dairy Science, 99(1): 746–757. Heidari, F., Dirandeh, E., Ansari-Pirsaraei Z. and Colazo, M.G. 2017. Modifications of the G6G timed-AI protocol improved pregnancy per AI and reduced pregnancy loss in lactating dairy cows. Animal, 11(11): 2002–2009. Hunter, R.H.F. 2005. The Fallopian Tubes in domestic mammals: How vital is their physiological activity? Reproduction Nutrition Development, 45(3): 281–290. Laven, R.A. and Peters, A.R. 2001. Gross morphometry of the bovine placentome during gestation. Reproduction in Domestic Animals, 36(6): 289–296. Lima, F.S., Bisinotto, R.S., Ribeiro, E.S., Ayres, H., Greco, L.F., Galvão, K.N., Risco, C.A., Thatcher, W.W. and Santos, J.E.P. 2012. Effect of one or three timed artificial inseminations before natural service on reproductive performance of lactating dairy cows not observed for detection of estrus. Theriogenology, 77(9): 1918–1927. Macmillan, K., Kastelic, J.P. and Colazo, M.G. 2018. Update on multiple ovulations in dairy cattle. Animals 8(5): 1–12. Moreira, F., Orlandi, C., Risco, C.A., Mattos, R., Lopes, F. and Thatcher, W.W. 2001. Effects of presynchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows. Journal of Dairy Science, 84(7): 1646–1659. Pursley, J.R., Mee, M.O. and Wiltbank, M.C. 1995. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 44(7): 915–923. Rivera, F.A., Mendonça, L.G.D., Lopes, G., Santos, J.E.P., Perez, R.V., Amstalden, M., Correa-Calderón, A. and Chebel, R.C. 2011. Reduced progesterone concentration during growth of the first follicular wave affects embryo quality but has no effect on embryo survival post transfer in lactating dairy cows. Reproduction, 141(3): 333–342. Santos, J.E.P., Rutigliano, H.M. and Filho, M.F.S. 2009. Risk factors for resumption of postpartum estrous cycles and embryonic survival in lactating dairy cows. Animal Reproduction Science, 110(3-4): 207–221. Santos, J.E.P., Thatcher, W.W., Chebel, R.C., Cerri, R.L.A. and Galvão, K.N. 2004. The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Animal Reproduction Science, 82–83(2004): 513–535. Sartori, R., Haughian, J.M., Shaver, R.D., Rosa, G.J.M. and Wiltbank, M.C. 2004. Comparison of ovarian function and circulating steroids in estrous cycles of Holstein heifers and lactating cows. Journal of Dairy Science, 87(4): 905–920. Shaham-Albalancy, A., Folman, Y., Kaim, M., Rosenberg, M. and Wolfenson, D. 2001. Delayed effect of low progesterone concentrations on bovine uterine PGF2α secretion in the subsequent oestrous cycle. Reproduction, 122(4): 643–648. Silva, C.C. and Knight, P.G. 2000. Effects of androgens, progesterone and their antagonists on the developmental competence of in vitro matured bovine oocytes. Journal of Reproduction and Fertility, 119(2): 261–269. Souza, A.H., Silva, E.P.B., Cunha, A.P., Gümen, A., Ayres, H., Brusveen, D.J., Guenther, J.N. and Wiltbank, M.C. 2011. Ultrasonographic evaluation of endometrial thickness near timed AI as a predictor of fertility in high-producing dairy cows. Theriogenology, 75(4): 722–733. Wiltbank, M.C., Baez, G.M., Garcia-Guerra, A., Toledo, M.Z., Monteiro, P.L.J., Melo, L.F., Ochoa, J.C., Santos, J.E.P. and Sartori, R. 2016. Pivotal periods for pregnancy loss during the first trimester of gestation in lactating dairy cows. Theriogenology 86(1), 239–253. Wiltbank, M., Lopez, H., Sartori, R., Sangsritavong, S. and Gümen, A. 2006. Changes in reproductive physiology of lactating dairy cows due to elevated steroid metabolism. Theriogenology, 65(1): 17–29. Wiltbank, M.C. and Pursley, J.R. 2014. The cow as an induced ovulator: Timed AI after synchronization of ovulation. Theriogenology, 81(1): 170–185. Wiltbank, M.C., Souza, A.H., Carvalho, P.D., Bender, R.W. and Nascimento, A.B. 2012. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reproduction, Fertility and Development, 24(1): 238–243. Wiltbank. M.C., Souza, A.H., Carvalho, P.D., Cunha, A.P., Giordano, J.O., Fricke, P.M., Baez, G.M. and Diskin, M.G. 2014. Physiological and practical effects of progesterone on reproduction in dairy cattle. Animal, 8(s1): 70–81. Young, C., Di Croce, F.A., Roper, D., Harris, J., Rohrbach, N., Wilkerson, J. and Schrick, F.N. 2010. Effect of reproductive tract size on conception rates in lactating dairy cows utilizing a reproductive tract scoring system. Reproduction, Fertility and Development 23(1), 119-119. | ||
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